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© 2021 American Chemical Society.Peroxynitrite (ONOO–) and glutathione (GSH), two unique reactive species, play an essential regulating role in the oxidation and antioxidation in the living body and are closely associated with various physiological and pathological processes, like cancer, cardiovascular disorders, diabetes, inflammation, Alzheimer's disease, and hepatotoxicity. Thus, it is crucial to study mitochondria ONOO–/GSH redox cycles by an effective molecular tool. In this work, a mitochondria–targeting and redox–reversible near–infrared (NIR) phosphorescent iridium complex, Ir–diol, has been synthesized and used for the detection and imaging of a cellular redox state by visualizing endogenous ONOO–/GSH content. Ir–diol shows excellent photophysical properties, including NIR emission (the maximum emissive wavelength for 704 nm, approximately) and high phosphorescent quantum yield (φ = 0.136) and exhibits high sensitivity and selectivity toward ONOO–/GSH redox cycles in aqueous solution and living cells. Therefore, these features, combined with low cytotoxicity and excellent cell permeability, enable probe Ir–diol to monitor the changes of the intracellular ONOO–/GSH level induced by drug both in vitro and in vivo.
