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© 2021 American Institute of Chemical EngineersThis contribution reports on a study using Purexa™–MQ multimodal anion–exchange (AEX) membranes for protein polishing at elevated solution conductivities. Dynamic binding capacities (DBC10) of bovine serum albumin (BSA), human immunoglobulins, and salmon sperm DNA (ss–DNA) are reported for various salt types, salt concentrations, flowrates, and pH. Using 1 mg/ml BSA, DBC10 values for Purexa™–MQ were >90 mg/ml at conductivities up to 15 mS/cm. The membranes maintained a high, salt–tolerant BSA DBC10 of 89.8 ± 2.7 (SD) over the course of 100 bind–elute cycles. Polishing studies with acidic and basic monoclonal antibodies at >2 kg/L loads showed that Purexa™–MQ had higher clearance of host cell proteins and aggregate species at high conductivity (13 mS/cm) and in the presence of phosphate than other commercial AEX media. Purexa™–MQ also had a high ss–DNA DBC10 of 50 mg/ml at conductivities up to 15 mS/cm, markedly outperforming other commercial products. In addition to the effectiveness of Purexa™–MQ for protein polishing at elevated solution conductivities, its unusually high binding capacity for ss–DNA indicates potential applications for plasmid DNA purification.
